Maki Tomoharu, Soh Nobuaki, Fukaminato Tuyoshi, Nakajima Hizuru, Nakano Koji, Imato Toshihiko
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
Anal Chim Acta. 2009 Apr 20;639(1-2):78-82. doi: 10.1016/j.aca.2009.02.044. Epub 2009 Mar 9.
A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical (OH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting OH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of OH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for OH. Spy-OH maxima excitation (lambda(exc)) and emission (lambda(em)) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of OH, especially for biosamples.
利用苝-3,4,9,10-四羧酸二酰亚胺和2,2,6,6-四甲基哌啶-1-氧基（TEMPO）合成了一种用于检测羟基自由基（OH）的新型荧光团连接氮氧化物。这种新型荧光探针，此处表示为Spy-OH（用于检测OH的燕尾苝衍生物），由于其结构中氮氧化物自由基的存在导致猝灭，其天然荧光被有效降低。在含有二甲基亚砜（DMSO）的介质中，当存在OH时，观察到荧光强度显著增加。除OH外，添加其他几种活性氧（ROS）时，荧光强度未观察到明显变化。Spy-OH的最大激发波长（λ(exc)）和发射波长（λ(em)）位于可见光区域（520/530 nm），因此，这种荧光探针有望用于简单、选择性地检测OH，特别是用于生物样品。
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