Cao Linying, Wu Qingfeng, Li Qiang, Shao Shijun, Guo Yong
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People's Republic of China.
University of Chinese Academy of Sciences, Beijing, 100039, People's Republic of China.
J Fluoresc. 2014 Mar;24(2):313-8. doi: 10.1007/s10895-013-1329-0. Epub 2013 Nov 28.
A rhodamine nitroxide probe was designed to detect the hydroxyl radical (·OH), which presented high selectivity for ·OH over other reactive oxygen species (ROS) and linear fluorescence response to ·OH produced by Fenton reaction. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by HPLC-UV/vis and HPLC-FLD both enhanced proportionally with the increase of ·OH concentration. The application of the probe in biological system was explored to trace the production of ·OH in cells under oxidative stress condition induced by rotenone which can inhibit the mitochondria respiratory chain complex I and we found that appropriate rotenone may induce the normal human liver cells (L02) and human hepatoma cells (HepG2) to produce ·OH at different degrees.
设计了一种罗丹明氮氧化物探针来检测羟基自由基（·OH），该探针对·OH的选择性高于其他活性氧（ROS），并且对芬顿反应产生的·OH具有线性荧光响应。通过HPLC-MS对产物进行检测，结果表明反应的主要产物是O-甲基羟胺，并且通过HPLC-UV/vis和HPLC-FLD测得的产物峰面积均随·OH浓度的增加而成比例增加。研究了该探针在生物体系中的应用，以追踪鱼藤酮诱导的氧化应激条件下细胞内·OH的产生，鱼藤酮可抑制线粒体呼吸链复合体I，我们发现适当浓度的鱼藤酮可不同程度地诱导正常人肝细胞（L02）和人肝癌细胞（HepG2）产生·OH。
