Scher William, Jing Yongkui, Lu Min, Bishop David F, Scher Barbara M
Department of Pathology, Mount Sinai Medical Center, 1194, One Gustave L. Levy Pl, New York, NY 10029, USA.
Arch Virol. 2009;154(5):895-8. doi: 10.1007/s00705-009-0372-2. Epub 2009 Apr 4.
Friend leukemia virus complex consists of a replication-competent virus plus one of two replication-incompetent viruses, spleen focus-forming virus anemia virus or spleen focus-forming virus polycythemia virus. The replication-incompetent viruses induce rapid malignant transformation of erythroid precursor cells. Transformed cell lines from mice infected with the complex can be induced to undergo erythrodifferentiation in vitro. However, lines containing the anemia-type virus require erythropoietin and another agent such as dimethyl sulfoxide for optimal erythrodifferentiation, whereas those containing the polycythemia-type virus do not require or respond to erythropoietin. Mice infected with the original Friend virus isolates were anemic, so sub-lines derived from these mice should be erythropoietin-dependent for induction of erythrodifferentiation. However, many of the widely studied sub-lines are erythropoietin-independent. In order to clarify this apparent anomaly, the genomes of viruses present in two commonly used erythropoietin-independent sub-lines were sequenced. Sequence analysis demonstrates that they contain the polycythemia-type virus and not the anemia-type virus.
Friend白血病病毒复合体由一种具有复制能力的病毒加上两种无复制能力的病毒之一组成，即脾集落形成病毒贫血病毒或脾集落形成病毒红细胞增多症病毒。无复制能力的病毒可诱导红系前体细胞快速发生恶性转化。感染该复合体的小鼠的转化细胞系可在体外被诱导进行红细胞分化。然而，含有贫血型病毒的细胞系需要促红细胞生成素和另一种试剂（如二甲基亚砜）来实现最佳的红细胞分化，而含有红细胞增多症型病毒的细胞系则不需要促红细胞生成素，也对其无反应。感染原始Friend病毒分离株的小鼠出现贫血，因此源自这些小鼠的亚系在诱导红细胞分化时应该依赖促红细胞生成素。然而，许多被广泛研究的亚系并不依赖促红细胞生成素。为了阐明这一明显的异常现象，对两个常用的不依赖促红细胞生成素的亚系中存在的病毒基因组进行了测序。序列分析表明，它们含有红细胞增多症型病毒，而不是贫血型病毒。
