A fractionation procedure for identifying novel proteins induced by chill stress in Arabidopsis thaliana.

作者信息

Sun Liwen, Wang Shichen, Xi Jinghui, Yang Shuo, Liu Xiangguo, Chai Xin, Xin Hongbao, An Baiyi, Hao Dongyun

机构信息

Key Lab for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130023, People's Republic of China.

出版信息

Biotechnol Lett. 2009 Aug;31(8):1289-95. doi: 10.1007/s10529-009-9985-5. Epub 2009 Apr 5.

DOI:10.1007/s10529-009-9985-5

PMID:19347259

Abstract

Extraction of plant proteins using typical extraction buffers leaves insoluble debris that cannot be investigated by conventional 2-DE technologies. In this paper, we present a scalable, off-line procedure for extraction of Arabidopsis thaliana homogenates that can be used in combination with both in-gel digestion and mass spectrometry. Based on sequential NaCl gradients and strong detergent fractionation, this new strategy allowed detection of 11 novel proteins from Arabidopsis thaliana that were altered in response to chilling stress.

摘要