Seung-Min Lee, Gil-Suk Yoon, Eun-Sang Yoo, Tae-Gyun Kwon, In-San Kim, Byung-Heon Lee
Department of Biochemistry and Cell Biology and Cell & Matrix Research Institute, Daegu, Korea.
Methods Mol Biol. 2009;512:355-63. doi: 10.1007/978-1-60327-530-9_20.
Selective delivery of drugs or imaging dyes to tumor is the central challenge for improving existing therapy and diagnosis of cancer. Phage display of random peptides has been used to identify homing peptides that are specific to tumor cells or tumor blood vessels. These homing peptides can be conjugated with imaging agents or therapeutic drugs and may be a promising tool for affinity-based targeted delivery of imaging agents and drugs. This chapter describes the procedures for phage display selection of peptides that selectively bind to bladder tumor cells and home to tumor tissues in vivo. It also describes the application of the tumor-specific peptide to the detection of bladder cancer cells in the patient urine.
将药物或成像染料选择性地递送至肿瘤是改善现有癌症治疗和诊断方法的核心挑战。随机肽的噬菌体展示已被用于鉴定对肿瘤细胞或肿瘤血管具有特异性的归巢肽。这些归巢肽可以与成像剂或治疗药物偶联，可能成为基于亲和力的成像剂和药物靶向递送的有前途的工具。本章介绍了通过噬菌体展示筛选能在体内选择性结合膀胱肿瘤细胞并归巢至肿瘤组织的肽的步骤。还描述了肿瘤特异性肽在检测患者尿液中膀胱癌细胞方面的应用。
