Schleicher Ulrike, Bogdan Christian
Institute of Clinical Microbiology, Immunology and Hygiene, University Clinic of Erlangen, Erlangen, Germany.
Methods Mol Biol. 2009;531:203-24. doi: 10.1007/978-1-59745-396-7_14.
Macrophages are not only host cells for many pathogens, but also fulfill several key functions in the innate and adaptive immune response, including the release of pro- and anti-inflammatory cytokines, the generation of organic and inorganic autacoids, the phagocytosis and killing of intracellular microorganisms or tumor cells, and the degradation and presentation of antigens. Several of these functions are shared by other immune cells, including dendritic cells, granulocytes, NK cells, and/or T lymphocytes. Thus, the analysis of macrophage functions in vitro using primary mouse cell populations requires standardized methods for the generation and culture of macrophages that guarantee high cell purity as well as the absence of stimulatory microbial contaminants. This chapter presents methodology to achieve these aims.
巨噬细胞不仅是许多病原体的宿主细胞，还在先天性和适应性免疫反应中发挥多种关键功能，包括释放促炎和抗炎细胞因子、生成有机和无机自分泌调节因子、吞噬和杀死细胞内微生物或肿瘤细胞，以及降解和呈递抗原。其中一些功能也为其他免疫细胞所共有，包括树突状细胞、粒细胞、自然杀伤细胞和/或T淋巴细胞。因此，使用原代小鼠细胞群体在体外分析巨噬细胞功能需要标准化的巨噬细胞生成和培养方法，以确保高细胞纯度以及不存在刺激性微生物污染物。本章介绍实现这些目标的方法。
