Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, People's Republic of China.
Mol Biol Rep. 2010 Mar;37(3):1347-53. doi: 10.1007/s11033-009-9514-6. Epub 2009 Apr 5.
Serum paraoxonase (PON1) plays an important role in protecting low-density lipoprotein against oxidative modification. In this study, the full-length cDNA sequence (1,416 bp) of porcine PON1 was cloned by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. It was found to contain a 1,068 bp open reading frame encoding a deduced protein of 355 amino acids with a calculated molecular weight of 40.02 kDa. The genomic structure and sequence of porcine PON1 were also analyzed using a bacterial artificial chromosome clone of a Chinese Erhualian pig. The porcine PON1 gene contained nine exons and eight introns spanning approximately 29 kb, and was located on chromosome nine between microsatellite markers SWR915 and SW944 by IMpRH mapping. Porcine PON1 was highly expressed in kidney, followed by liver, lung and small intestine, expressed at an extremely low level in heart, and was hardly expressed in spleen, lymph, muscle of the anterior limb, cerebrum, fat, cerebellum or hypothalamus.
血清对氧磷酶（PON1）在保护低密度脂蛋白免受氧化修饰方面发挥着重要作用。本研究通过反转录聚合酶链反应和快速扩增 cDNA 末端，克隆了猪 PON1 的全长 cDNA 序列（1416bp）。结果发现，它包含一个 1068bp 的开放阅读框，编码一个由 355 个氨基酸组成的推导蛋白，计算分子量为 40.02kDa。还使用中国二花脸猪的细菌人工染色体克隆分析了猪 PON1 的基因组结构和序列。猪 PON1 基因包含 9 个外显子和 8 个内含子，跨越约 29kb，通过 IMpRH 图谱定位在第 9 号染色体上，位于微卫星标记 SWR915 和 SW944 之间。猪 PON1 在肾脏中高度表达，其次是肝脏、肺和小肠，在心脏中表达水平极低，在脾脏、淋巴、前肢肌肉、大脑、脂肪、小脑或下丘脑几乎不表达。
