Identification of HLA-A*02-B*46 haplotype allele variant in Guangdong Han populations on the basis of PCR-SBT.

BACKGROUND

The HLA-A02-B46 haplotype is one of most frequent haplotypes among Guangdong Han populations. To explore the characteristics of the HLA-A02-B46 haplotype in Guangdong Han populations, the genetic polymorphism of HLA-A02-B46 haplotype was analysed by PCR-SBT in our study.

FINDINGS

Among 88 samples with the homozygotes for HLA-A02-B46 in the low resolution, 4 different alleles for A02 (A0201, A0203, A0206, A0207) and 1 allele for B46 (B4601) were identified by PCR-SBT. Among them, the A0207 allele was the predominant allele. Inversely, among the samples with HLA-A2-B46(-), six alleles were detected for A02 (A020101, A0203, A0205, A0206 and A0207), and the A0201 allele was predominant. On the other hand, the HLA-A02-B46 haplotype presented moderate heterozygosis (32.95%). In addition, the linkage with DRB1 was analysed in HLA-A2-B46 haplotype, and there existed 10 alleles with DRB1. With the low resolution for DRB1, the other 10 DRB1 alleles all linked with the HLA-A02-B46 haplotype except for DRB101, DRB110, and DRB117. Moreover, we found eight alleles of DRB1 in the HLA-A0207-B4601 haplotype.

CONCLUSION

The polymorphism distribution of the A02 allele between the HLA-A02-B46 and HLA-A02-B46(-) haplotypes among the Guangdong Han populations provides useful information for research on unrelated hematopoietic stem cell transplantation (UHSCT), anthropology, and disease association for populations with the HLA-A02-B*46 haplotype.