第13章 2Fe-2S线粒体外膜蛋白米托内（mitoNEET）的定位与功能
Chapter 13 Localization and function of the 2Fe-2S outer mitochondrial membrane protein mitoNEET.
作者信息
Wiley Sandra E, Rardin Matthew J, Dixon Jack E
机构信息
Department of Pharmacology, University of California, San Diego, La Jolla, California, USA.
出版信息
Methods Enzymol. 2009;456:233-46. doi: 10.1016/S0076-6879(08)04413-3.
MitoNEET is an integral protein of the outer mitochondrial membrane and is the flagship of a small family of proteins whose hallmark is the presence of a CDGSH domain. Initially annotated as a zinc finger, the CDGSH domain actually binds a redox-active 2Fe-2S cluster, giving mitoNEET the distinction of being the first 2Fe-2S protein identified in the outer membrane of mitochondria. This chapter describes methods for isolating mitochondrial membrane fractions that are enriched in mitoNEET, generating constructs for the expression of recombinant mitoNEET protein and analyzing the 2Fe-2S cluster of mitoNEET in vitro.
线粒体衍生的电子传递体（MitoNEET）是线粒体外膜的一种整合蛋白，是一小类蛋白质家族的核心成员，其特征是存在一个CDGSH结构域。CDGSH结构域最初被注释为锌指结构，实际上它结合一个具有氧化还原活性的2Fe-2S簇，这使MitoNEET成为在线粒体外膜中鉴定出的首个2Fe-2S蛋白。本章介绍了分离富含MitoNEET的线粒体膜组分的方法、构建用于表达重组MitoNEET蛋白的载体以及在体外分析MitoNEET的2Fe-2S簇的方法。
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