使用微流控装置控制噬菌体展示的筛选严格性。

Controlling the selection stringency of phage display using a microfluidic device.

作者信息

Liu Yanli, Adams Jonathan D, Turner Kelisha, Cochran Frank V, Gambhir Sanjiv Sam, Soh H Tom

机构信息

Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.

出版信息

Lab Chip. 2009 Apr 21;9(8):1033-6. doi: 10.1039/b820985e. Epub 2009 Mar 3.

DOI:10.1039/b820985e

PMID:19350081

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

摘要

我们报告了利用微流控技术进行噬菌体筛选，并证明在我们的微流控磁分离器（MMS）中对洗涤严格度的精确控制直接影响分离肽序列的多样性。可重复产生的磁力和流体力允许实现可控的洗涤条件，从而使所选肽序列能够快速趋同。这些发现可能为开发用于亲和试剂快速体外定向进化的自动化微系统提供基础。