Alvisi Nicolò, de Vries Renko
Physical Chemistry and Soft Matter, Wageningen University & Research, Stippeneng 4, 6708WE, Wageningen, the Netherlands.
Mater Today Bio. 2023 Feb 15;19:100580. doi: 10.1016/j.mtbio.2023.100580. eCollection 2023 Apr.
Over the past decades, solid-binding peptides (SBPs) have found multiple applications in materials science. In non-covalent surface modification strategies, solid-binding peptides are a simple and versatile tool for the immobilization of biomolecules on a vast variety of solid surfaces. Especially in physiological environments, SBPs can increase the biocompatibility of hybrid materials and offer tunable properties for the display of biomolecules with minimal impact on their functionality. All these features make SBPs attractive for the manufacturing of bioinspired materials in diagnostic and therapeutic applications. In particular, biomedical applications such as drug delivery, biosensing, and regenerative therapies have benefited from the introduction of SBPs. Here, we review recent literature on the use of solid-binding peptides and solid-binding proteins in biomedical applications. We focus on applications where modulating the interactions between solid materials and biomolecules is crucial. In this review, we describe solid-binding peptides and proteins, providing background on sequence design and binding mechanism. We then discuss their application on materials relevant for biomedicine (calcium phosphates, silicates, ice crystals, metals, plastics, and graphene). Although the limited characterization of SBPs still represents a challenge for their design and widespread application, our review shows that SBP-mediated bioconjugation can be easily introduced into complex designs and on nanomaterials with very different surface chemistries.
在过去几十年中，固相结合肽（SBPs）在材料科学领域得到了广泛应用。在非共价表面修饰策略中，固相结合肽是一种简单且通用的工具，可用于将生物分子固定在各种固体表面。特别是在生理环境中，SBPs可以提高杂化材料的生物相容性，并为生物分子的展示提供可调节的特性，同时对其功能的影响最小。所有这些特性使得SBPs在诊断和治疗应用中制造仿生材料方面具有吸引力。特别是，药物递送、生物传感和再生治疗等生物医学应用已受益于SBPs的引入。在此，我们综述了关于固相结合肽和固相结合蛋白在生物医学应用中的最新文献。我们关注那些调节固体材料与生物分子之间相互作用至关重要的应用。在本综述中，我们描述了固相结合肽和蛋白，提供了序列设计和结合机制的背景知识。然后，我们讨论了它们在与生物医学相关的材料（磷酸钙、硅酸盐、冰晶、金属、塑料和石墨烯）上的应用。尽管SBPs的表征有限仍然是其设计和广泛应用的一个挑战，但我们的综述表明，SBP介导的生物共轭可以很容易地引入到复杂设计中以及具有非常不同表面化学性质的纳米材料上。
