Zhang Yong, Gu Zhong-ping, Zhou Yong-an, Wang Yun-jie
Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Apr;25(4):341-3, 347.
To observe the effect of inhabitation of VEGF expression by RNA interference on the therapy of lung cancer and to obtain a novel strategy of lung cancer therapy by RNA interference.
A segment of VEGF-siRNA was synthesized according to the code of siRNA design. The constructed pSilencer-3.1-VEGF vectors were stably transfected into A549 cell line. Then the expression of VEGF was detected in stable transfectant A549 cell line by TR-PCR and Western blot. Meanwhile, the growth of stable transfectant A549 cell line and the angiogenesis of endothelial cells were studied. The tumor volume was detected in stable transfectant A549 bearing mice.
The expression of VEGF in stable transfectant A549 cell line was obviously inhibited by pSilencer-3.1-VEGF-2. Although it did not inhibit the growth of A549, it decreased the angiogenesis of endothelial cells. In vivo, compared with control group, the tumor in the pSilencer-3.1-VEGF-2-A549 bearing mice(P<0.05)growth was obviously at a slow, and the survival obviously lengthened in the pSilencer-3.1-VEGF-2-A549 bearing mice(P<0.05).
A pSilencer-3.1-VEGF vector has been successfully constructed. pSilencer-3.1-VEGF-2 can silence the expression of VEGF in A549. It can inhibit the angiogenesis of endothelial cells in vitro and the tumor growth and lengthen the survival of the bearing mice in vivo.
观察RNA干扰抑制VEGF表达对肺癌治疗的影响，以获得一种新的肺癌RNA干扰治疗策略。
根据siRNA设计编码合成一段VEGF-siRNA。将构建好的pSilencer-3.1-VEGF载体稳定转染至A549细胞系。然后通过TR-PCR和Western blot检测稳定转染的A549细胞系中VEGF的表达。同时，研究稳定转染的A549细胞系的生长及内皮细胞的血管生成情况。检测稳定转染A549细胞系的荷瘤小鼠的肿瘤体积。
pSilencer-3.1-VEGF-2可明显抑制稳定转染的A549细胞系中VEGF的表达。虽然它未抑制A549的生长，但可降低内皮细胞的血管生成。在体内，与对照组相比，pSilencer-3.1-VEGF-2-A549荷瘤小鼠的肿瘤生长明显缓慢（P<0.05），且pSilencer-3.1-VEGF-2-A549荷瘤小鼠的生存期明显延长（P<0.05）。
成功构建了pSilencer-3.1-VEGF载体。pSilencer-3.1-VEGF-2可使A549中VEGF的表达沉默。它在体外可抑制内皮细胞的血管生成，在体内可抑制肿瘤生长并延长荷瘤小鼠的生存期。
