Liu Y J, Chen Y P, Jin P K, Wang X C
Key Laboratory of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Anaerobe. 2009 Oct;15(5):214-8. doi: 10.1016/j.anaerobe.2009.03.007. Epub 2009 Apr 5.
Bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in Changqing Oil field in China were investigated by 16S rRNA denaturing gradient gel electrophoresis (DGGE) analysis followed by gene cloning and sequencing. DGGE profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to Ochrobactrum sp. and Stenotrophomonas sp. were detected in all crude oil and oil field water samples. Bacteria related to Burkholderia sp., Brevundimonas sp., and Propionibacterium sp. were detected in the crude oil samples but not in water samples. Bacteria related to Hippea sp., Acidovorax sp., Arcobacter sp., Pseudomonas sp., Thiomicrospira sp., Brevibacterium sp., Tissierella sp. and Peptostreptococcus sp. were detected in the water samples but not in crude oil samples. Using an archaea-specific primer set, methanogens related to Methanomicrobials and Methanosarcinales were found in water samples but not in crude oil samples. The comparability of the microbial communities in the water and crude oil phase during the period of oil gathering and transferring process was 83.3% and 88.2%, respectively, indicating a stable structure of the microbial communities.
采用16S rRNA变性梯度凝胶电泳（DGGE）分析，随后进行基因克隆和测序，对中国长庆油田集输系统原油和油田采出水样品中的细菌群落进行了研究。DGGE图谱显示，水样中的细菌群落远比原油样品中的丰富，并且在所有原油和油田水样中均检测到与慢生根瘤菌属（Ochrobactrum sp.）和嗜麦芽窄食单胞菌属（Stenotrophomonas sp.）相关的细菌。在原油样品中检测到与伯克霍尔德菌属（Burkholderia sp.）、短波单胞菌属（Brevundimonas sp.）和丙酸杆菌属（Propionibacterium sp.）相关的细菌，但在水样中未检测到。在水样中检测到与希佩菌属（Hippea sp.）、嗜酸菌属（Acidovorax sp.）、弓形杆菌属（Arcobacter sp.）、假单胞菌属（Pseudomonas sp.）、硫微螺菌属（Thiomicrospira sp.）、短杆菌属（Brevibacterium sp.）、蒂西埃拉菌属（Tissierella sp.）和消化链球菌属（Peptostreptococcus sp.）相关的细菌，但在原油样品中未检测到。使用古菌特异性引物组，在水样中发现了与甲烷微菌目（Methanomicrobials）和甲烷八叠球菌目（Methanosarcinales）相关的产甲烷菌，但在原油样品中未发现。集输过程中，水相和原油相中微生物群落的相似性分别为83.3%和88.2%，表明微生物群落结构稳定。
