从辐射脉菌菌株BP - 11 - 2中纯化并鉴定一种可使真菌黑色素脱色的胞外漆酶。
Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin.
作者信息
Kaneko Shuji, Cheng Minyi, Murai Hiroshi, Takenaka Shinji, Murakami Shuichiro, Aoki Kenji
机构信息
Department of Applied Biological Chemistry, Graduate School of Agricultural Science, Kobe University, Rokko, Kobe 657-8501, Japan.
出版信息
Biosci Biotechnol Biochem. 2009 Apr 23;73(4):939-42. doi: 10.1271/bbb.80740. Epub 2009 Apr 7.
Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH2-terminal amino acid sequences.
从森林土壤中分离得到的辐射状嗜皮菌菌株BP-12-2可使真菌黑色素脱色，并在培养过程中产生一种胞外漆酶。该酶经纯化后达到均一状态并进行了特性鉴定。在300nm以上的紫外区域或可见光区域未显示吸收带。它在底物特异性、动力学参数和氨基末端氨基酸序列方面与已报道的漆酶不同。
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