Chernykh A, Myasoedova N, Kolomytseva M, Ferraroni M, Briganti F, Scozzafava A, Golovleva L
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino, Moscow Region, Russia.
J Appl Microbiol. 2008 Dec;105(6):2065-75. doi: 10.1111/j.1365-2672.2008.03924.x.
To isolate and characterize the laccase isoforms from S. ochraceum 1833 - a new active producer of high extracellular laccase activity.
Three laccase isoforms (laccases I, II and III) with 57.5, 59.5 and 63 kDa molecular masses respectively were purified from S. ochraceum 1833 and in contrast to the known laccases had strongly pronounced absorption at 611 nm with molar extinction coefficients ranging from 7170 to 7830 mol(-1) l cm(-1). All isoforms showed maximal activity with ABTS at low pH (<or=2) and temperatures in the range 70-80 degrees C, were stable for long time of incubation at high temperature (60-80 degrees C) and at pH values ranging from 2 to 6. Laccase II showed a higher activity and wider substrate specificity. N-terminal amino acid sequence analysis of the purified laccase II (VQIGPVTDLH) showed 80% identity with the N-terminal amino acid sequence of laccase from Lentinula edodes [Appl Microbiol Biotechnol 60 (2002) 327].
Elevated temperature optima, high thermo- and pH-stabilities, the broad substrate specificity of the isoforms make the laccases from S. ochraceum 1833 a suitable model for biotechnological processes proceeding at high temperatures.
For the first time, new basidiomycete strain S. ochraceum was reported as a producer of novel thermostable, pH stable, acidophilic laccases with unusual spectral properties.
从赭黄丝膜菌1833中分离并鉴定漆酶同工酶，该菌株是一种新的具有高细胞外漆酶活性的活跃生产者。
从赭黄丝膜菌1833中纯化出三种漆酶同工酶（漆酶I、II和III），分子量分别为57.5、59.5和63 kDa，与已知漆酶不同的是，它们在611 nm处有强烈的吸收峰，摩尔消光系数在7170至7830 mol⁻¹ l cm⁻¹之间。所有同工酶在低pH值（≤2）和70 - 80℃温度下对ABTS表现出最大活性，在高温（60 - 80℃）和pH值为2至6的范围内长时间孵育时稳定。漆酶II表现出更高的活性和更广泛的底物特异性。纯化的漆酶II的N端氨基酸序列分析（VQIGPVTDLH）显示与香菇漆酶的N端氨基酸序列有80%的同源性[应用微生物学与生物技术60（2002）327]。
最适温度升高、高热稳定性和pH稳定性以及同工酶广泛的底物特异性使得赭黄丝膜菌1833的漆酶成为高温生物技术过程的合适模型。
首次报道新的担子菌菌株赭黄丝膜菌是新型耐热、pH稳定、嗜酸且具有异常光谱特性的漆酶的生产者。
