Willemze Annemieke J, Bakker Astrid C, von dem Borne Peter A, Bajema Ingeborg M, Vossen Jaak M
Department of Paediatrics, Division of Immunology, Haematology, Oncology and Bone Marrow Transplantation and Autoimmune Diseases, Leiden University Medical Centre, Leiden, The Netherlands.
Transplantation. 2009 Apr 15;87(7):1096-101. doi: 10.1097/TP.0b013e31819d340f.
Recent evidence indicates that bone marrow-derived cells contribute to endothelial and epithelial cell renewal in recipients of an allogeneic stem-cell transplantation (SCT). Controversy remains on the biological significance of these donor-derived cells.
This study investigated the occurrence of endothelial and epithelial cell chimerism in relation to the conditioning regimen, time interval after SCT, and development of acute and chronic graft-versus-host disease (GVHD). Fifty-five skin biopsy samples from 35 female patients transplanted with a male donor were screened for donor-derived endothelial and epithelial cells using in situ hybridization for Y chromosomes in combination with immunohistochemical cell-marking techniques.
Endothelial cell chimerism was found in 25% of the biopsies and increased in time after SCT. Its appearance was increased in patients with acute GVHD more than 2 weeks before biopsy. Epithelial cell chimerism was found in 85% of the biopsies. Appearance of epithelial cell chimerism was not correlated with the time interval after SCT or with tissue damage caused by GVHD.
From these results, we conclude that donor-derived endothelial cell chimerism results from repair of damaged endothelium and maintenance of vascular homeostasis. In contrast, epithelial cell chimerism follows a more uniform pattern of engraftment, not influenced by tissue damage.
最近的证据表明，在同种异体干细胞移植（SCT）受者中，骨髓来源的细胞有助于内皮细胞和上皮细胞的更新。这些供体来源细胞的生物学意义仍存在争议。
本研究调查了内皮细胞和上皮细胞嵌合体的发生情况，及其与预处理方案、SCT后的时间间隔以及急性和慢性移植物抗宿主病（GVHD）发展的关系。对35例接受男性供体移植的女性患者的55份皮肤活检样本，采用Y染色体原位杂交结合免疫组化细胞标记技术，筛选供体来源的内皮细胞和上皮细胞。
25%的活检样本中发现了内皮细胞嵌合体，且在SCT后随时间增加。在活检前2周以上发生急性GVHD的患者中，其出现率更高。85%的活检样本中发现了上皮细胞嵌合体。上皮细胞嵌合体的出现与SCT后的时间间隔或GVHD引起的组织损伤无关。
从这些结果中，我们得出结论，供体来源的内皮细胞嵌合体是由受损内皮的修复和血管稳态的维持导致的。相比之下，上皮细胞嵌合体遵循更一致的植入模式，不受组织损伤的影响。
