Brydie J R, Wogelius R A, Boult S, Merrifield C M, Vaughan D J
Alberta Research Council, Edmonton, Alberta, Canada.
Biofouling. 2009;25(5):463-72. doi: 10.1080/08927010902913351.
Biofilm development on mineral surfaces and related changes in surface reactivity were studied using batch and flow through experiments. An artificial groundwater was used as the primary nutrient medium, Pseudomonas aeruginosa (PAO1) was the model microbial organism and 'mineral' surfaces were kept as simple as possible by using glass or a polished quartz tile. Experiments were also completed with very low concentrations (100 mg l(-1)) of iron, Fe(2+ ), in the solution. In situ confocal laser scanning microscopy of developing colonies during the live growth phase, and of thick, mature biofilms, revealed only sporadic coverage of biofilm cells and associated polymers at the 'mineral-microbe interface'. Imaging and analysis of biofilm-conditioned surfaces doped with Fe(2+ )-rich solutions allowed the locus and form of Fe-rich mineral precipitation to be determined and show that biological surface components can cause mineral precipitation from dilute dissolved species which might otherwise remain in solution.
利用分批实验和流通实验研究了矿物表面生物膜的形成以及表面反应性的相关变化。使用人工地下水作为主要营养培养基，铜绿假单胞菌（PAO1）作为模型微生物，通过使用玻璃或抛光石英砖使“矿物”表面尽可能简单。实验还在溶液中含有极低浓度（100 mg l(-1)）的铁（Fe(2+)）的条件下完成。在活生长阶段对正在形成的菌落以及厚的成熟生物膜进行原位共聚焦激光扫描显微镜观察，结果显示在“矿物 - 微生物界面”处生物膜细胞和相关聚合物仅有零星覆盖。对用富含Fe(2+)的溶液处理过的生物膜表面进行成像和分析，能够确定富铁矿物沉淀的位置和形态，并表明生物表面成分可导致稀溶解物种形成矿物沉淀，否则这些物种可能会留在溶液中。
