Roffler S R, Yu M H, Chen M R, Chen S C, Hsu C T, Yeh M Y
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1991 Feb;24(1):19-29.
A new murine monoclonal IgG3 antibody (Mab 1H10) was developed with specificity for human cervical carcinoma and several other tumor types. Antibody reactivity against a panel of tumor cell lines was examined by indirect immunofluorescence and quantified by flow cytometry. Mab 1H10 reacted with cervical, colorectal and bladder carcinoma cells and to a lesser extent melanoma and hepatocellular carcinoma cells but did not react with human fibroblasts, lymphocytes or RBCs. Mab 1H10, as assessed by immunohistochemical staining, bound 40/97 cervical carcinoma tissue samples, 8/16 colorectal carcinoma samples as well as a population of osteogenic sarcoma and lung, ovarian and bladder carcinoma tissues. Mab 1H10 did not react with any normal tissue or cell samples tested including cervix, ovary, breast, liver, colon, bladder, lung, spleen, cerebrum, lymphocytes or RBCs. Mab 1H10 may be useful for the targeting of drugs, toxins or radioisotopes to cervical carcinoma in humans.
一种新的鼠源单克隆IgG3抗体（单克隆抗体1H10）被开发出来，它对人宫颈癌及其他几种肿瘤类型具有特异性。通过间接免疫荧光法检测了该抗体对一组肿瘤细胞系的反应性，并通过流式细胞术进行定量分析。单克隆抗体1H10与宫颈癌细胞、结肠直肠癌细胞和膀胱癌细胞发生反应，对黑色素瘤细胞和肝癌细胞的反应较弱，但不与人成纤维细胞、淋巴细胞或红细胞发生反应。通过免疫组织化学染色评估，单克隆抗体1H10与40/97例宫颈癌组织样本、8/16例结肠直肠癌样本以及一定数量的骨肉瘤、肺癌、卵巢癌和膀胱癌组织结合。单克隆抗体1H10与所检测的任何正常组织或细胞样本均无反应，包括子宫颈、卵巢、乳腺、肝脏、结肠、膀胱、肺、脾脏、大脑、淋巴细胞或红细胞。单克隆抗体1H10可能有助于将药物、毒素或放射性同位素靶向人体内的宫颈癌。
