使用微芯片电泳、圆二色性和一维核磁共振对含有GGA重复序列的RNA适体在离子条件下进行结构研究。
Structural studies of an RNA aptamer containing GGA repeats under ionic conditions using microchip electrophoresis, circular dichroism, and 1D-NMR.
作者信息
Nishikawa Fumiko, Murakami Kazuyoshi, Matsugami Akimasa, Katahira Masato, Nishikawa Satoshi
机构信息
Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
出版信息
Oligonucleotides. 2009 Jun;19(2):179-90. doi: 10.1089/oli.2008.0167.
Nuclear magnetic resonance (NMR) studies have shown that RNA/DNA oligomers with GGA repeat sequences contain unique G-quadruplex structures in the presence of K(+) or Na(+) ions. In this study, we used microchip electrophoresis to study the structure of an RNA aptamer against bovine prion protein that possessed four GGA-triplet repeats (wt2). We analyzed the structural changes and characterized dimer formation of the aptamer. Mutational, circular dichroism, and one-dimensional NMR studies of wt2 revealed that K(+) ions induce wt2 to assume a thermostable dimer in an intramolecular G-quadruplex with parallel orientation.
核磁共振（NMR）研究表明，具有GGA重复序列的RNA/DNA寡聚物在存在K(+)或Na(+)离子的情况下包含独特的G-四链体结构。在本研究中，我们使用微芯片电泳来研究一种针对牛朊病毒蛋白的RNA适体的结构，该适体具有四个GGA三联体重复序列（wt2）。我们分析了该适体的结构变化并对其二聚体形成进行了表征。对wt2的突变、圆二色性和一维NMR研究表明，K(+)离子诱导wt2在分子内以平行取向的G-四链体中形成热稳定的二聚体。
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