酶源对重组细胞色素P450 3A4与NADPH-细胞色素P450还原酶联合催化咪达唑仑1'-羟化活性的影响。
Effects of enzyme sources on midazolam 1'-hydroxylation activity catalyzed by recombinant cytochrome P450 3A4 in combination with NADPH-cytochrome P450 reductase.
作者信息
Emoto Chie, Murayama Norie, Yamazaki Hiroshi
机构信息
Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194-8583, Japan.
出版信息
Drug Metab Lett. 2008 Aug;2(3):190-2. doi: 10.2174/187231208785425737.
PMID:19356092
Abstract
The influence of the enzyme source such as phospholipid and expression ratio of NADPH-cytochrome P450 reductase on midazolam 1'-hydroxylation activity of cytochrome P450 3A4 was investigated in reconstituted systems and in membranes using bicistronic expressions. Preferable ratio of P450 reductase over human P450 3A4 would be a determinant factor for the drug metabolism studies.
摘要
在重组系统和使用双顺反子表达的膜中，研究了磷脂等酶源以及NADPH-细胞色素P450还原酶的表达比例对细胞色素P450 3A4咪达唑仑1'-羟化活性的影响。P450还原酶与人P450 3A4的适宜比例将是药物代谢研究的一个决定性因素。
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