Brans Richard, Akhrameyeva Natali V, Yao Feng
Department of Surgery, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, USA.
J Invest Dermatol. 2009 Oct;129(10):2470-9. doi: 10.1038/jid.2009.86. Epub 2009 Apr 9.
CJ9-gD is a novel herpes simplex virus (HSV) type 1 recombinant virus that is completely replication-defective, expresses high-levels of HSV-1 major antigen glycoprotein D (gD), and can function in trans to inhibit replication of wild-type HSV-1 and HSV-2 in co-infected cells. Here, we show that immunization with CJ9-gD elicits strong and long-lasting humoral and Th1-like cellular immune responses against both HSV-1 and HSV-2. Mice immunized with CJ9-gD exhibited significant reductions in the extent and duration of intravaginal replication of challenge HSV-1 and HSV-2 compared with mock-immunized controls, and were completely protected from local or systemic herpetic disease after intravaginal challenge with wild-type HSV-1 or HSV-2.
CJ9-gD是一种新型的1型单纯疱疹病毒(HSV)重组病毒,它完全缺乏复制能力,能高水平表达HSV-1主要抗原糖蛋白D(gD),并且在共感染细胞中可通过反式作用抑制野生型HSV-1和HSV-2的复制。在此,我们表明用CJ9-gD免疫可引发针对HSV-1和HSV-2的强烈且持久的体液免疫和Th1样细胞免疫反应。与 mock免疫对照相比,用CJ9-gD免疫的小鼠在感染HSV-1和HSV-2后,阴道内病毒复制的程度和持续时间显著降低,在用野生型HSV-1或HSV-2进行阴道内攻击后,可完全免受局部或全身性疱疹疾病的侵害。