Shen Fei, Li Zhi-tao, Wu Yi-ming, Wu Wei-dong
Department of Occupational Health and Environmental Health, Public Health College, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Aug;26(8):453-6.
To detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro.
AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment.
The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.
AP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.
在体外检测并验证A549细胞系中激活蛋白-1（AP-1）与p53基因启动子区域的结合情况。
采用染色质免疫沉淀（ChIP）分析，使用c-jun特异性抗体检测AP-1与p53基因启动子区域的结合，并通过PCR扩增其基因特异性DNA片段。
在c-jun抗体免疫沉淀的DNA片段中发现了p53基因特异性片段。
AP-1在A549细胞中与p53基因启动子区域结合，并调节其表达。
