Zhang X H, Zhong X, Zhou Y M, Du H M, Wang T
College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China.
Poult Sci. 2009 May;88(5):959-66. doi: 10.3382/ps.2008-00512.
The objective of this study was to compare the effect of 2 esters of alpha-tocopherol, all-rac-alpha-tocopherol acetate and RRR-alpha-tocopherol succinate (d-alpha-TOS) on growth and immunity in broiler chicks. Three hundred twenty 1-d-old commercial Arbor Acres broilers were randomly distributed to 4 treatments, each of which had 8 pens of 10 chicks per pen. Birds in the control group were fed with the diets supplemented with 30 mg/kg of all-rac-alpha-tocopherol acetate or the basal diet with d-alpha-TOS supplementation at 10 mg/kg (TOS1 group), 30 mg/kg (TOS2 group), and 50 mg/ kg (TOS3 group), respectively, for 42 d. The results showed that there was no significant difference (P > 0.05) in BW gain, feed intake, or G:F among the treatments. Significant positive correlations existed between dietary supplemental alpha-TOS levels and plasma (R(2) = 0.9831, P < 0.01) or hepatic (R(2) = 0.9336, P < 0.05) alpha-tocopherol concentrations and a negative correlation with plasma (R(2) = 0.9487, P < 0.05) or hepatic (R(2) = -0.9901, P = 0.0518) malondialdehyde levels. The concentrations of serum glutathione (GSH) were highest at 50 mg/kg at 42 d of age (P < 0.05), and hepatic GSH was significantly higher at 30 and 50 mg/kg compared with the other groups. Marked enhancement of splenic T- and B-lymphocyte proliferation occurred in group TOS3 as compared with the other groups. The study suggests that the immunoenhancement effect observed in broilers fed additional d-alpha-TOS between 30 and 50 mg/kg might result from increased retention of alpha-tocopherol and reduction in lipid peroxidation, as evidenced by the decrease in malondialdehyde and the increase in GSH.
本研究的目的是比较两种α-生育酚酯，即消旋-α-生育酚醋酸酯和RRR-α-生育酚琥珀酸酯（d-α-TOS）对肉仔鸡生长和免疫的影响。320只1日龄的商品艾维茵肉仔鸡被随机分为4组处理，每组8个栏，每栏10只鸡。对照组的鸡分别饲喂添加30 mg/kg消旋-α-生育酚醋酸酯的日粮或添加10 mg/kg（TOS1组）、30 mg/kg（TOS2组）和50 mg/kg（TOS3组）d-α-TOS的基础日粮，持续42天。结果表明，各处理组之间的体重增加、采食量或料重比均无显著差异（P>0.05）。日粮中添加α-TOS水平与血浆（R² = 0.9831，P<0.01）或肝脏（R² = 0.9336，P<0.05）中α-生育酚浓度呈显著正相关，与血浆（R² = 0.9487，P<0.05）或肝脏（R² = -0.9901，P = 0.0518）中丙二醛水平呈负相关。血清谷胱甘肽（GSH）浓度在42日龄时以50 mg/kg组最高（P<0.05），肝脏GSH在30和50 mg/kg组显著高于其他组。与其他组相比，TOS3组脾脏T淋巴细胞和B淋巴细胞增殖显著增强。该研究表明，在饲喂30至50 mg/kg额外d-α-TOS的肉仔鸡中观察到的免疫增强作用可能是由于α-生育酚保留增加和脂质过氧化减少，丙二醛减少和GSH增加证明了这一点。
