Yamaji Masahiro, Shudo Jutaro
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Int Immunopharmacol. 2009 Jul;9(7-8):984-9. doi: 10.1016/j.intimp.2009.04.004. Epub 2009 Apr 9.
Epicutaneous application of a contact sensitizer 2, 4-dinitro-1-chlorobenzene (DNCB) is known to cause an inflammatory response and a hapten-specific T cell activation. We developed a highly stable gel formulation for DNCB which is easy to apply to the skin. The present study focused on whether topical application of DNCB gel produced immunomodulation with prophylactic effects against lethal herpes simplex virus infection (HSV) type 1. Our results indicated that topical treatment with DNCB gel induced proliferation of lymph node cells, T cells as well as antigen presenting cells, the production of cytokines, especially IFN-gamma, and cutaneous inflammatory response comparable to that from freshly prepared DNCB in olive oil. Repeated topical treatment of DNCB gel resulted in prophylactic effect against lethal infection with herpes simplex virus type 1 (HSV-1). In mice repeatedly treated with DNCB gel and infected with HSV, a significant increase in serum IFN-gamma was observed. These results suggest that topical DNCB functions as a potent immunomodulator, activating anti-viral defense mechanisms.
已知经皮应用接触性致敏剂2,4-二硝基-1-氯苯（DNCB）会引发炎症反应和半抗原特异性T细胞活化。我们开发了一种用于DNCB的高度稳定的凝胶制剂，该制剂易于涂抹于皮肤。本研究聚焦于局部应用DNCB凝胶是否能产生免疫调节作用，并对致死性1型单纯疱疹病毒感染（HSV）具有预防效果。我们的结果表明，用DNCB凝胶进行局部治疗可诱导淋巴结细胞、T细胞以及抗原呈递细胞增殖，产生细胞因子，尤其是γ干扰素，并且引发的皮肤炎症反应与在橄榄油中新鲜制备的DNCB所引发的反应相当。重复局部应用DNCB凝胶可对致死性1型单纯疱疹病毒（HSV-1）感染产生预防作用。在用DNCB凝胶反复治疗并感染HSV的小鼠中，观察到血清γ干扰素显著增加。这些结果表明，局部应用DNCB可作为一种有效的免疫调节剂，激活抗病毒防御机制。
