Hoa X D, Kirk A G, Tabrizian M
Department of Biomedical Engineering, McGill University, Montréal, Canada.
Biosens Bioelectron. 2009 Jun 15;24(10):3043-8. doi: 10.1016/j.bios.2009.03.021. Epub 2009 Mar 24.
In this report, nano-gratings with guided adsorption of biomolecules are investigated as new transducer elements or biointerfaces for surface plasmon resonance biosensor technologies. SPR biosensors are of particular interest due to the interaction between the electromagnetic fields and periodic nano-structures. In this article, sensitivity enhancement is demonstrated for a surface plasmon resonance interface, in a Kretschmann's configuration, featuring nano-gratings combined with nano-patterned immobilization of surface bioreceptors. The fabrication of this enhanced biointerface is demonstrated using a combination of metal lift-off and self-assembled monolayers. Rigorous coupled-wave analyses point to an increase in SPR angular response for the immobilization of surface bioreceptors onto areas of the nano-corrugated surface exhibiting high electromagnetic field intensity. Experimental measurements of the immobilization of anti-TNF-alpha antibody as a model bioreceptor using an imaging-SPR technique show a 3 times increase in angular resonance response from nano-grating surfaces with functionalized mesas compared to a planar surface or to a uniformly functionalized nano-grating surface. Furthermore, results also show an increased detection of TNF-alpha due to the increased accessibility to the adsorbed bioreceptors on the nano-gratings.
在本报告中，研究了具有生物分子导向吸附功能的纳米光栅，将其作为表面等离子体共振生物传感器技术的新型换能器元件或生物界面。由于电磁场与周期性纳米结构之间的相互作用，表面等离子体共振生物传感器备受关注。在本文中，展示了在Kretschmann配置下，结合纳米光栅与表面生物受体的纳米图案固定化的表面等离子体共振界面的灵敏度增强。利用金属剥离和自组装单分子层相结合的方法展示了这种增强型生物界面的制备过程。严格耦合波分析表明，将表面生物受体固定在具有高电磁场强度的纳米波纹表面区域时，表面等离子体共振角响应会增加。使用成像表面等离子体共振技术对作为模型生物受体的抗TNF-α抗体进行固定化的实验测量表明，与平面表面或均匀功能化的纳米光栅表面相比，具有功能化台面的纳米光栅表面的角共振响应增加了3倍。此外，结果还表明，由于纳米光栅上吸附的生物受体的可及性增加，对TNF-α的检测也有所增加。
