Hoa X D, Martin M, Jimenez A, Beauvais J, Charette P, Kirk A, Tabrizian M
Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, Que., Canada.
Biosens Bioelectron. 2008 Dec 1;24(4):976-81. doi: 10.1016/j.bios.2008.07.069. Epub 2008 Aug 9.
Surfaces featuring nano-structures and biochemical patterns are increasingly developed as novel and superior substrates for biosensors and assays. Metallic periodic nano-structures have been studied for their unique optical properties and in particular their ability to support surface plasmon waves. Here we present a new nano-structuring approach based on gentle metal lift-off process coupled with self-assembled surface chemistry for the fabrication of a zeroth-order 400nm period metallic grating with differentiated surface chemistries on the mesas and troughs. The approach, using terminated self-assembled monolayers, creates versatile functionalized substrates allowing the precise deposition of complex biomolecular structures. We use this technique to perform the guided deposition of a three-dimensional polyelectrolyte multilayer structure and the patterned adsorption of quantum dots. Finally, we demonstrate that scanning near-field optical microscopy, used in conjuncture with atomic force microscopy and scanning electron microscopy, is an ideal tool for the characterization of this nano-structured surface as it provides a complete chemical, topographical and optical image of the surface. This ability to pattern and locally measure the surface properties is likely to have an important impact on the design of novel and optimized biointerfaces and transducers for biosensors.
具有纳米结构和生化图案的表面正日益被开发为用于生物传感器和检测的新型优质基底。金属周期性纳米结构因其独特的光学性质，尤其是其支持表面等离子体波的能力而受到研究。在此，我们展示了一种基于温和金属剥离工艺并结合自组装表面化学的新型纳米结构化方法，用于制造具有在台面和凹槽上具有不同表面化学性质的零阶400nm周期金属光栅。该方法使用末端自组装单分子层，创建了多功能的功能化基底，允许精确沉积复杂的生物分子结构。我们使用该技术进行三维聚电解质多层结构的引导沉积和量子点的图案化吸附。最后，我们证明，与原子力显微镜和扫描电子显微镜结合使用的扫描近场光学显微镜是表征这种纳米结构表面的理想工具，因为它提供了表面的完整化学、形貌和光学图像。这种对表面性质进行图案化和局部测量的能力可能会对新型和优化的生物界面以及生物传感器换能器的设计产生重要影响。
