探寻第五种碱基：DNA甲基化分析技术
Hunting for the 5th base: Techniques for analyzing DNA methylation.
作者信息
Ammerpohl Ole, Martín-Subero José I, Richter Julia, Vater Inga, Siebert Reiner
机构信息
Institute of Human Genetics, Christian-Albrechts University Kiel, University Hospital Schleswig-Holstein Campus Kiel, Germany.
出版信息
Biochim Biophys Acta. 2009 Sep;1790(9):847-62. doi: 10.1016/j.bbagen.2009.02.001. Epub 2009 Feb 9.
BACKGROUND
In the recent past large progress has been made in the analysis of the epigenome, the entirety of epigenetic modifications, and its meaning for the implementation of the genetic code. Besides histone modifications and miRNA expression, DNA methylation is one of the key players in the field of epigenetics, involved in numerous regulatory processes.
METHODS
In the present review we focus on methods for the analysis of DNA methylation patterns and present an overview about techniques and basic principles available for this purpose.
RESULTS AND GENERAL SIGNIFICANCE
We here discuss advantages and disadvantages of various methods and their feasibility for specific tasks of DNA methylation analysis.
背景
最近，在表观基因组分析方面取得了重大进展，表观基因组是指所有表观遗传修饰及其在遗传密码实现中的意义。除了组蛋白修饰和微小RNA表达外，DNA甲基化是表观遗传学领域的关键因素之一，参与众多调控过程。
方法
在本综述中，我们重点关注DNA甲基化模式的分析方法，并概述为此目的可用的技术和基本原理。
结果与普遍意义
我们在此讨论各种方法的优缺点及其在DNA甲基化分析特定任务中的可行性。
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