Zachary Andrea A, Lucas Donna P, Detrick Barbara, Leffell Mary S
Departments of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hum Immunol. 2009 Jul;70(7):496-501. doi: 10.1016/j.humimm.2009.04.001. Epub 2009 Apr 11.
Substances occurring naturally in the sera of patients can interfere with Luminex antibody assays, causing increased background and changes in antibody specificity. We present data on the effectiveness of hypotonic dialysis (HD) or dithiothreitol (DTT) treatment in eliminating this interference. HD significantly increased reaction strength of positive control beads and reduced reaction strength of negative control beads. HD also improved specificity identification, determination of donor-specific antibody (DSA) strength, and crossmatch predictability compared with values in untreated serum. DTT also increased the reaction strength of positive control beads, but in most cases, further increased reactivity of negative control beads. DTT improved crossmatch predictability but to a lesser extent than did HD and may differ with specificities defined in other assays. Because interference is frequently observed in sera from highly sensitized patients, it is important to recognize and eliminate interference in Luminex antibody assays for accurate and meaningful test interpretation.
患者血清中天然存在的物质会干扰Luminex抗体检测，导致背景增加和抗体特异性改变。我们展示了关于低渗透析（HD）或二硫苏糖醇（DTT）处理消除这种干扰有效性的数据。HD显著增加了阳性对照微珠的反应强度，并降低了阴性对照微珠的反应强度。与未处理血清中的值相比，HD还改善了特异性鉴定、供体特异性抗体（DSA）强度的测定以及交叉配型的可预测性。DTT也增加了阳性对照微珠的反应强度，但在大多数情况下，进一步增加了阴性对照微珠的反应性。DTT改善了交叉配型的可预测性，但程度低于HD，并且可能因其他检测中定义的特异性而异。由于在高度致敏患者的血清中经常观察到干扰，因此在Luminex抗体检测中识别和消除干扰对于准确和有意义的检测解读很重要。
