Sugiyama Tetsuya, Mashima Yukihiko, Yoshioka Yuriko, Oku Hidehiro, Ikeda Tsunehiko
Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City 569-8686, Japan.
Arch Ophthalmol. 2009 Apr;127(4):454-9. doi: 10.1001/archophthalmol.2008.606.
To determine whether subconjunctival injection of unoprostone isopropyl alters changes in the topography and blood flow of the optic disc induced by endothelin 1 (ET-1) in rabbits.
From April 1, 2005, to April 28, 2006, we injected ET-1 (20 pmol) intravitreally into rabbits twice per week for 4 weeks. The observation period was 8 weeks. The first group received an intravitreal injection of ET-1 followed by a subconjunctival injection of unoprostone (0.12%, 50 microL). The second group received the same amount of ET-1 followed by a subconjunctival injection of the vehicle of unoprostone. The third group received the intravitreal vehicle of ET-1. The blood flow and topography of the optic nerve head (ONH) were measured by laser speckle flowgraphy and confocal scanning ophthalmoscopy, respectively. The number of cells in the retinal ganglion cell layer and inner nuclear layer was determined histologically.
We found that ET-1 decreased the ONH blood flow, decreased the cells in the ganglion cell layer and inner nuclear layer, enlarged the cup area of the ONH, and reduced the rim area of the ONH. When unoprostone was given with ET-1, no such changes occurred.
Unoprostone can suppress the effects of ET-1 on the circulation and topography of the ONH.
Unoprostone could be a candidate for treating eyes with ischemic ONH.
确定结膜下注射异丙酸乌诺前列酮是否能改变内皮素1（ET-1）诱导的兔视盘地形图和血流变化。
从2005年4月1日至2006年4月28日，我们每周两次向兔玻璃体内注射ET-1（20皮摩尔），共4周。观察期为8周。第一组接受玻璃体内注射ET-1，随后结膜下注射异丙酸乌诺前列酮（0.12%，50微升）。第二组接受相同剂量的ET-1，随后结膜下注射异丙酸乌诺前列酮的溶媒。第三组接受ET-1的玻璃体内溶媒。分别通过激光散斑血流图和共焦扫描检眼镜测量视神经乳头（ONH）的血流和地形图。通过组织学方法确定视网膜神经节细胞层和内核层中的细胞数量。
我们发现ET-1降低了ONH血流，减少了神经节细胞层和内核层中的细胞数量，扩大了ONH的杯状区域，并减小了ONH的边缘区域。当异丙酸乌诺前列酮与ET-1同时使用时，未出现此类变化。
异丙酸乌诺前列酮可抑制ET-1对ONH循环和地形图的影响。
异丙酸乌诺前列酮可能是治疗缺血性ONH眼病的候选药物。
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