Escalante Maryana, Blum Christian, Cesa Yanina, Otto Cees, Subramaniam Vinod
Biophysical Engineering Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Langmuir. 2009 Jun 16;25(12):7019-24. doi: 10.1021/la900223f.
We report for the first time the directed assembly and characterization of FRET pairs on micrometer patterned surfaces. We used visible fluorescent proteins expressing a hexahistidine affinity tag as component molecules for the construction of the FRET constructs, where His(6)-EGFP served as donor fluorophore and His(6-)DsRed-FT as the acceptor. We created 2D and 3D structures that exhibit fluorescence resonance energy transfer at the interfaces between the donor and acceptor patterns in the lateral or axial directions, respectively. We quantitatively visualized the energy transfer by multiparameter optical microscopy.
我们首次报道了在微米级图案化表面上定向组装和表征荧光共振能量转移（FRET）对。我们使用表达六聚组氨酸亲和标签的可见荧光蛋白作为构建FRET构建体的组成分子，其中His(6)-EGFP作为供体荧光团，His(6-)DsRed-FT作为受体。我们创建了二维和三维结构，分别在横向或轴向的供体和受体图案之间的界面处表现出荧光共振能量转移。我们通过多参数光学显微镜对能量转移进行了定量可视化。
