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[采用酶联免疫吸附测定法检测粪便标本中的溶组织内阿米巴]

[Detection of Entamoeba histolytica in stool specimens with the ELISA method].

作者信息

Mengeloğlu Firat Zafer, Aktaş Elif, Külah Canan, Cömert Füsun Beğendik

机构信息

Zonguldak Karaelmas Universitesi Tip Fakültesi, Tibbi Mikrobiyoloji Anabilim Dali, Zonguldak, Turkey.

出版信息

Turkiye Parazitol Derg. 2009;33(1):1-3.

Abstract

In this study, carried out between November 2006 and September 2007, amebic cysts were observed in 44 (0.37%) out of a total of 1720 stool specimens which were examined by direct microscopy. Entamoeba histolytica specific antigen was investigated with ELISA in the specimens in which cysts were observed. Specific antigen was detected in 26 (59.1%) of these specimens. The diagnosis of amoebiasis for the patients whose ELISA tests were positive was confirmed and the appropriate therapy was immediately begun. This prediagnosis was abandoned for patients whose test results were "negative". Because of the low sensitivity of direct microscopy in confirming the prediagnosis of amoebiasis, it is necessary to perform ELISA on the specimens in order to determine whether the patient should be treated or to prevent patients from being given an unnecessary treatment.

摘要

在这项于2006年11月至2007年9月期间开展的研究中,在通过直接显微镜检查的总共1720份粪便标本中,有44份(0.37%)观察到阿米巴囊肿。对观察到囊肿的标本采用酶联免疫吸附测定法(ELISA)检测溶组织内阿米巴特异性抗原。在这些标本中有26份(59.1%)检测到特异性抗原。对ELISA检测呈阳性的患者确诊为阿米巴病,并立即开始适当治疗。对于检测结果为“阴性”的患者则放弃这种预诊断。由于直接显微镜检查在确诊阿米巴病预诊断方面的敏感性较低,有必要对标本进行ELISA检测,以确定患者是否应接受治疗,或防止患者接受不必要的治疗。

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