Alver Oktay, Topaç Tuncay, Töre Okan
Uludağ Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Bursa,Türkiye.
Turkiye Parazitol Derg. 2015 Sep;39(3):185-9. doi: 10.5152/tpd.2015.3727.
This study aims to compare the performance of Native-Lugol examination and EIA Antigen Detection Test using stool samples obtained from patients diagnosed as clinical gastroenteritis and submitted to the Parasitology Laboratory in Uludağ University between January 2010 and February 2011.
The stool samples taken from 116 patients and sent to the laboratory of parasitology from various clinics including outpatient services have been investigated using Native-Lugol examination and EIA Antigen Detection Kit (Wampole® E. histolytica II Techlab®, Inc., Blacksburg, Virginia) methods on all the samples.
In one of 116 stool samples (%0,86), E. histolytica/E. dispar cysts and/or trophozoites were detected by using direct microscobic (nativ-lugol) method. E. histolytica specific antigen was detected in 34 (29.3%) out of the sample set, and the patients were given adequate treatment. The highest rate of E. histolytica specific antigen positivity were observed in 11-19 age group.
On account of the fact that the sensitivity of direct microscopy is quite low, it is concluded that, from the viewpoint of preventing the amebiasis suspected patients from false diagnosis and hence from receiving inadequate treatment, the use of the ELISA method is more appropriate and advantageous, as it is cost effective and does not require highly qualified staff.
本研究旨在比较自然卢戈氏碘液检查法与酶免疫分析抗原检测试验的性能，所使用的粪便样本取自2010年1月至2011年2月间在乌鲁达大学寄生虫学实验室被诊断为临床肠胃炎的患者。
从包括门诊服务在内的各个诊所采集了116名患者的粪便样本并送至寄生虫学实验室，所有样本均采用自然卢戈氏碘液检查法和酶免疫分析抗原检测试剂盒（Wampole®溶组织内阿米巴II，Techlab®公司，弗吉尼亚州布莱克斯堡）方法进行检测。
在116份粪便样本中的1份（0.86%）中，通过直接显微镜检查（自然卢戈氏碘液法）检测到了溶组织内阿米巴/迪斯帕包囊和/或滋养体。在样本组中，有34份（29.3%）检测到了溶组织内阿米巴特异性抗原，这些患者均接受了适当治疗。溶组织内阿米巴特异性抗原阳性率最高的是11 - 19岁年龄组。
鉴于直接显微镜检查的灵敏度相当低，得出结论：从防止疑似阿米巴病患者被误诊从而避免接受不充分治疗的角度来看，酶联免疫吸附测定法的使用更为合适且具有优势，因为它具有成本效益且不需要高素质的工作人员。
