聚（环氧乙烷）-聚（环氧丙烷）-聚（环氧乙烷）三嵌段共聚物的自发囊泡形成

Spontaneous vesicle formation of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer.

作者信息

Chen Shu, Yang Bin, Guo Chen, Ma Jun-He, Yang Liang-Rong, Liang Xiangfeng, Hua Chao, Liu Hui-Zhou

机构信息

Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

J Phys Chem B. 2008 Dec 11;112(49):15659-65. doi: 10.1021/jp8019039.

DOI:10.1021/jp8019039

PMID:19367948

Abstract

A novel method has been developed to prepare vesicles from aqueous solutions of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer, by adding anionic surfactant sodium dodecyl sulfate (SDS) and inorganic salt NaF. As determined by TEM and dynamic light scattering (DLS) measurements, the average diameter of vesicles is about 800 nm having 50 nm outer shell thickness. Identifying hydrophobic interactions between the block copolymers and the microenvironments around the vesicles using FTIR, 1H NMR, and fluorescence spectroscopy techniques revealed the vesicle formation mechanism. The spontaneously formed vesicles were further cross-linked by converting the terminal hydroxyl groups of block copolymers into aldehydes, and then chemically bridging the polymer chains by the reaction between aldehydes and diamine compounds. The cross-linked vesicles are proved much more stable than free vesicles even at higher dilutions. The obtained vesicles with good stability and biocompatibility are promising candidates for widespread applications.

摘要

已开发出一种新方法，通过添加阴离子表面活性剂十二烷基硫酸钠（SDS）和无机盐NaF，从聚（环氧乙烷）-聚（环氧丙烷）-聚（环氧乙烷）三嵌段共聚物的水溶液中制备囊泡。通过透射电子显微镜（TEM）和动态光散射（DLS）测量确定，囊泡的平均直径约为800nm，外壳厚度为50nm。使用傅里叶变换红外光谱（FTIR）、核磁共振氢谱（1H NMR）和荧光光谱技术鉴定嵌段共聚物与囊泡周围微环境之间的疏水相互作用，揭示了囊泡的形成机制。通过将嵌段共聚物的末端羟基转化为醛，然后通过醛与二胺化合物之间的反应使聚合物链化学桥连，使自发形成的囊泡进一步交联。即使在更高的稀释度下，交联囊泡也比游离囊泡稳定得多。所获得的具有良好稳定性和生物相容性的囊泡有望广泛应用。