Holle G E
Gastroenterology Research Laboratory, Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University, Munich, Germany.
Gastroenterology. 1991 Nov;101(5):1264-73. doi: 10.1016/0016-5085(91)90076-w.
Tritiated thymidine was administered IP to rats that had been exposed to benzalkonium chloride in the duodenum, jejunum, and ileum, resulting in neuronal ablation. Epithelial cell proliferation and migration were studied 21 and 7 days after treatment. Significant hyperplasia and hypertrophy of the villi and crypts was seen from day 7 on. This was half as pronounced as that of the muscle layer, whose maximal percent increase was not seen until day 21. In the crypt, the proliferation had increased significantly (65% 3H index corrected) and its zone had expanded proportionally to the total crypt depth. After an average of 36 hours in the ileum (48 hours in normal rats), labeled cells reached the tip of the lengthened villi, reflecting significantly accelerated migration. Concerning the distributional pattern of the labeled cells in the crypt, a nonsignificant shift to the lower two thirds of the crypt could be distinguished. From this the author concludes that treatment with benzalkonium chloride influences the proliferation and migration of the epithelial cells in the treated area. These alterations may result from loss of the myenteric plexus, but other factors cannot be excluded.
将氚标记的胸腺嘧啶核苷经腹腔注射给予十二指肠、空肠和回肠暴露于苯扎氯铵的大鼠，导致神经元消融。在治疗后21天和7天研究上皮细胞增殖和迁移。从第7天起可见绒毛和隐窝显著增生和肥大。这一程度是肌层的一半，肌层的最大百分比增加直到第21天才出现。在隐窝中，增殖显著增加（校正后的3H指数为65%），其区域与隐窝总深度成比例扩大。在回肠平均36小时后（正常大鼠为48小时），标记细胞到达延长绒毛的顶端，反映迁移显著加速。关于标记细胞在隐窝中的分布模式，可以区分出向隐窝下三分之二的不显著转移。作者由此得出结论，苯扎氯铵治疗会影响治疗区域上皮细胞的增殖和迁移。这些改变可能是由于肌间神经丛的丧失，但其他因素也不能排除。
