Abdullaev S A, Guliaeva N A, Bezlepkin V G, Gaziev A I
Radiats Biol Radioecol. 2009 Jan-Feb;49(1):21-8.
We defined the mutations in mtDNA of X-irradiated mice brair using mismatch-specific endonuclease (CEL I-nuclease method) and by temporal temperature gradient gel electrophoresis (TTGE-technique). The comparison of the received by both methods, allows to conclude, that CEL I-nuclease method gives more qualitative results, than TTGE-technique. Moreover, CEL I-nuclease method is more sensitive, in contrast with TTGE-technique. The CEL I-nuclease method allows simultaneously to conduct the analysis of big amount of sample DNA, to get the reproducible results. It does not require complex equipment and economical. The analysis of mutations in mtDNA of brain of X-irradiated mice by CEL I-nuclease method has shown, that the amount of mutant copies mtDNA is essentially reduced (in 2-3 times) with 8 up to 28 days of the post-radiation period. However the amount mtDNA copies in brain tissue of the irradiated animals is remains during all post radiation time without change though lower, concerning given control group. The results permit the suggestion that mutant mtDNA copies are eliminated from the tissues of irradiated animals in the post-radiation period.
我们使用错配特异性内切酶（CEL I核酸酶法）并通过时间温度梯度凝胶电泳（TTGE技术）定义了X射线照射小鼠大脑线粒体DNA中的突变。对两种方法所得结果的比较表明，CEL I核酸酶法比TTGE技术给出的定性结果更好。此外，与TTGE技术相比，CEL I核酸酶法更灵敏。CEL I核酸酶法能够同时对大量样本DNA进行分析，获得可重复的结果。它不需要复杂的设备且经济实惠。通过CEL I核酸酶法对X射线照射小鼠大脑线粒体DNA中的突变进行分析表明，在辐射后8至28天期间，突变型线粒体DNA拷贝数显著减少（减少2至3倍）。然而，尽管与给定的对照组相比更低，但在整个辐射后期间，受照射动物脑组织中线粒体DNA拷贝数保持不变。这些结果表明，突变型线粒体DNA拷贝在辐射后时期从受照射动物的组织中被清除。
