School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk, Republic of Korea.
Bioresour Technol. 2010 Jan;101 Suppl 1:S23-8. doi: 10.1016/j.biortech.2009.03.054. Epub 2009 Apr 14.
A pilot-scale upflow anaerobic sludge blanket (UASB) treating swine wastewater was operated for 382 days to evaluate the process performance and methanogenic population dynamics. A real-time quantitative PCR (QPCR) was used to detect and quantify the 16S rRNA gene concentrations of the domain Archaea, the four methanogenic orders, and the two aceticlastic families. Extended intervals of consistently stable and efficient wastewater treatment with a final hydraulic retention time (HRT) of 3.5 days were sustained. A high abundance of hydrogenotrophic methanogens was observed, with Methanobacteriales as the major group, suggesting that hydrogenotrophic methanogenesis, with the syntrophic oxidation of volatile fatty acids (VFAs), was a major route of methane formation. This phenomenon was mainly attributed to the high ammonium concentration in swine wastewater, which has a severe inhibitory effect mainly on aceticlastic methanogens. Although there was no significant growth of Methanosaetaceae, its abundance contributed to the formation and maintenance of granule.
采用升流式厌氧污泥床（UASB）处理猪废水进行中试，以评估工艺性能和产甲烷种群动态。使用实时定量 PCR（QPCR）检测和定量检测古菌域、四个产甲烷目和两个乙酸裂解科的 16S rRNA 基因浓度。最终水力停留时间（HRT）为 3.5 天，稳定高效的废水处理可维持较长时间。观察到氢营养型产甲烷菌的丰度很高，其中 Methanobacteriales 为主要群体，表明氢营养型产甲烷作用与挥发性脂肪酸（VFAs）的共氧化是甲烷形成的主要途径。这种现象主要归因于猪废水中高浓度的铵，其对乙酸营养型产甲烷菌具有严重的抑制作用。尽管 Methanosaetaceae 的生长没有明显增加，但它的丰度有助于颗粒的形成和维持。
