School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Republic of Korea.
Water Res. 2010 Sep;44(17):4900-7. doi: 10.1016/j.watres.2010.07.029. Epub 2010 Jul 16.
Qualitative and quantitative molecular analysis techniques were used to determine associations between differences in methanogenic microbial communities and the efficiency of batch anaerobic digesters. Two bioreactors were initially seeded with anaerobic sludge originating from a local municipal wastewater treatment plant and then supplemented with swine wastewater. Differences were observed in the total amount of methane produced in the two bioreactors (7.9L/L, and 4.5L/L, respectively). To explain these differences, efforts were taken to characterize the microbial populations present using a PCR-based DGGE analysis with methanogenic primer and probe sets. The groups Methanomicrobiales (MMB), Methanobacteriales (MBT), and Methanosarcinales (MSL) were detected, but Methanococcales (MCC) was not detected. Following this qualitative assay, real-time PCR was used to investigate quantitative differences in the populations of these methanogenic orders. MMB was found to be the dominant order present and its abundance patterns were different in the two digesters. The population profiles of the other methanogenic groups also differed. Through redundancy analysis, correlations between the concentrations of the different microbes and chemical properties such as volatile fatty acids were calculated. Correlations between MBT and MSL populations and chemical properties were found to be consistent in both digesters, however, differences were observed in the correlations between MMB and propionate. These results suggest that interactions between populations of MMB and other methanogens affected the final methane yield, despite MMB remaining the dominant group overall. The exact details of why changes in the MMB community caused different profiles of methane production could not be ascertained. However, this research provides evidence that microbial behavior is important for regulating the performance of anaerobic processes.
采用定性和定量分子分析技术来确定产甲烷微生物群落的差异与批式厌氧消化器效率之间的关联。两个生物反应器最初用来自当地城市污水处理厂的厌氧污泥接种，然后用猪废水进行补充。两个生物反应器中产生的甲烷总量存在差异（分别为 7.9L/L 和 4.5L/L）。为了解释这些差异，我们努力使用基于 PCR 的 DGGE 分析，使用产甲烷引物和探针组来表征存在的微生物种群。检测到 Methanomicrobiales (MMB)、Methanobacteriales (MBT) 和 Methanosarcinales (MSL) 组，但未检测到 Methanococcales (MCC)。在进行定性分析后，使用实时 PCR 研究这些产甲烷菌目种群的定量差异。发现 MMB 是主要存在的目，其丰度模式在两个消化器中有所不同。其他产甲烷菌组的种群分布也不同。通过冗余分析，计算了不同微生物浓度与挥发性脂肪酸等化学性质之间的相关性。发现 MBT 和 MSL 种群与化学性质之间的相关性在两个消化器中是一致的，然而，在 MMB 和丙酸盐之间的相关性观察到差异。这些结果表明，尽管 MMB 总体上仍然是主要群体，但 MMB 种群与其他产甲烷菌之间的相互作用会影响最终的甲烷产量。无法确定 MMB 群落变化导致甲烷产生不同模式的确切原因。然而，这项研究提供了证据，表明微生物行为对于调节厌氧过程的性能非常重要。
