Orlov Iu N, Rebane W N
Zh Evol Biokhim Fiziol. 2009 Jan-Feb;45(1):75-9.
In this work, dynamics of uptake ofp-aminohippurate (PAH) by basolateral membrane vesicles isolated from rat kidney proximal tubules was studied. The uphill PAH transport into the basolateral membrane vesicles in the presence of alpha-ketoglutarate and Na+-gradient was shown. Based on mathematical model of symport and antiport cooperation the mechanism of energy coupling of PAH transport by the way of exchanger with Na+-dicarboxylate symport is discussed. Based on comparison of our own and literature data, the data analysis shows adequacy of the proposed mathematical model to describe the symport and antiport cooperation. This model was demonstrated to enable estimation of re-orientation probability of the empty anion exchanger (without substrate) from one membrane side to the other.
在这项工作中，研究了从大鼠肾近端小管分离的基底外侧膜囊泡对对氨基马尿酸（PAH）的摄取动力学。结果表明，在α-酮戊二酸和Na+梯度存在的情况下，PAH可向基底外侧膜囊泡进行上坡转运。基于同向转运和反向转运协同作用的数学模型，讨论了PAH通过与Na+-二羧酸同向转运体交换的方式进行能量偶联的机制。通过比较我们自己的数据和文献数据，数据分析表明所提出的数学模型足以描述同向转运和反向转运的协同作用。该模型被证明能够估计空阴离子交换体（无底物）从膜的一侧重新定向到另一侧的概率。
