微相分离对聚合物表面蛋白质抗性的影响。

Effect of microphase separation on the protein resistance of a polymeric surface.

作者信息

Ma Chunfeng, Hou Yi, Liu Shi, Zhang Guangzhao

机构信息

Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China.

出版信息

Langmuir. 2009 Aug 18;25(16):9467-72. doi: 10.1021/la900669p.

DOI:10.1021/la900669p

PMID:19371047

Abstract

Segmented polyurethanes (PUs) containing poly(ethylene glycol) (PEG), poly(propylene glycol), or poly(dimethylsiloxane) soft segments have been prepared by two-step condensation polymerization. Atom force microscopy observation in air and solution indicates that the segmented PU forms a microphase separation on the surface. By use of quartz crystal microbalance with dissipation and surface plasmon resonance, we have investigated the adsorption of fibrinogen, bovine serum albumin, and lysozyme on a surface constructed by such a PU in aqueous solution in real time. Our results reveal that the protein resistance of the PUs arises from the hydrated PEG segments instead of microphase separation.

摘要

通过两步缩聚反应制备了含有聚乙二醇（PEG）、聚丙二醇或聚二甲基硅氧烷软段的嵌段聚氨酯（PU）。在空气和溶液中进行的原子力显微镜观察表明，嵌段PU在表面形成微相分离。利用具有耗散功能的石英晶体微天平以及表面等离子体共振技术，我们实时研究了纤维蛋白原、牛血清白蛋白和溶菌酶在由这种PU构建的水溶液表面上的吸附情况。我们的结果表明，PU的蛋白质抗性源于水合的PEG链段而非微相分离。