采用高效液相色谱-蒸发光散射检测法同时定量测定妥布霉素和硫酸多粘菌素。
Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection.
作者信息
Clarot I, Storme-Paris I, Chaminade P, Estevenon O, Nicolas A, Rieutord A
机构信息
SRSMC, Nancy Université CNRS, France.
出版信息
J Pharm Biomed Anal. 2009 Aug 15;50(1):64-7. doi: 10.1016/j.jpba.2009.03.013. Epub 2009 Mar 25.
A rapid and simple method for the simultaneous determination of tobramycin and colistin sulphate in a pharmaceutical formulation by reversed phase HPLC and evaporative light scattering detection is described. Chromatographic separation was carried out in gradient mode using a Zorbax SB C18 column (150mmx4mm, 3.5microm) with mobile phases of acetonitrile and water containing trifluoroacetic at 1ml/min. The method was validated using methodology described by the International Conference of Harmonization. The method was shown to be specific, precise, accurate and linear. Real samples were analyzed to demonstrate the applicability of the chromatographic method in a routine use.
本文描述了一种通过反相高效液相色谱法和蒸发光散射检测同时测定药物制剂中妥布霉素和硫酸黏菌素的快速简便方法。色谱分离采用梯度模式，使用Zorbax SB C18柱（150mm×4mm，3.5μm），流动相为乙腈和含1ml/min三氟乙酸的水。该方法按照国际协调会议描述的方法进行了验证。结果表明该方法具有特异性、精密度、准确性和线性。通过分析实际样品证明了该色谱方法在常规应用中的适用性。
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