采用蒸发光散射检测的高效液相色谱法同时定量测定不同环糊精和甘膦树脂。
Simultaneous quantification of different cyclodextrins and Gantrez by HPLC with evaporative light scattering detection.
作者信息
Agüeros M, Campanero M A, lrache J M
机构信息
Centro Galénico, Universidad de Navarra, Aptdo. 177, C/ Irunlarrea s/n, 31080 Pamplona, Navarra, Spain.
出版信息
J Pharm Biomed Anal. 2005 Sep 15;39(3-4):495-502. doi: 10.1016/j.jpba.2005.04.034.
A rapid and simple HPLC method with evaporative scattering detection (ELSD) has been developed for the separation and quantitation of beta-cyclodextrin (beta-CD), 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) and poly(methyl vinyl ether-co-maleic anhydride) (Gantrez). Separation was carried out on a Zorbax Eclipse XDB-Phenyl narrow bore column, with water-acetonitrile in gradient elution as mobile phase at a flow-rate of 0.25 ml/min. Polyethylenglycol 6000 was used as internal standard. The limit of quantification was of about 0.2 mg/ml for cyclodextrins and 0.05 mg/ml for Gantrez. The precision did not exceed 7%. This method was successfully applied to the rapid analysis of CD-Gantrez nanoparticle conjugates without interference from other components of the formulation.
已开发出一种采用蒸发光散射检测（ELSD）的快速简便的高效液相色谱法，用于分离和定量β-环糊精（β-CD）、2-羟丙基-β-环糊精（2-HP-β-CD）和聚（甲基乙烯基醚-马来酸酐）（Gantrez）。在Zorbax Eclipse XDB-苯基窄径柱上进行分离，以水-乙腈梯度洗脱作为流动相，流速为0.25 ml/min。聚乙二醇6000用作内标。环糊精的定量限约为0.2 mg/ml，Gantrez的定量限为0.05 mg/ml。精密度不超过7%。该方法成功应用于CD-Gantrez纳米颗粒缀合物的快速分析，不受制剂中其他成分的干扰。
Zotero 插件