Sreeramulu D, Reddy C Vijaya Kumar, Raghunath M
Endocrinology and Metabolism Division, National Institute of Nutrition, Hyderabad 500 604, A.P, India.
Indian J Biochem Biophys. 2009 Feb;46(1):112-5.
Plant foods are important due to their antioxidant activity (AOA) attributed to the phenolics which are known to protect organisms against harmful effects of oxygen radicals. However, information on antioxidant activity of Indian plant foods is scanty. Therefore, the present study evaluated the AOA of cereals, millets, pulses and legumes, commonly consumed in India and assessed the relationship with their total phenolic content (TPC). AOA was assessed by DPPH (2,2-Diphenyl-1-picryl hydrazyl) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay and reducing power. DPPH scavenging activity ranged from 0.24 and 1.73 mg/g, whereas FRAP ranged from 16.21 to 471.71 micromoles/g. Finger millet (Eleusine cora cana) and Rajmah (Phaseolus vulgaris) had the highest FRAP 471.71, 372.76 and DPPH scavenging activity 1.73, 1.07. Similar trends were observed with reducing power. Among cereals and legumes, Finger millet (Ragi) and black gram dhal (Phaseolus mungo Roxb) had the highest TPC, the values being 373 and 418 mg/100 g respectively, while rice (Oryza sativa) and green gram dhal (Phaseolus aureus Roxb) showed the least (47.6 and 62.4 mg/100 g). In the present study, FRAP (r = 0.91) and reducing power (r = 0.90) showed significant correlation with TPC in cereals and millets, but not in pulses and legumes. The results suggest that TPC contributes significantly to the AOA of Indian cereals and millets.
植物性食物很重要,因为其酚类物质具有抗氧化活性(AOA),已知这些酚类物质可保护生物体免受氧自由基的有害影响。然而,关于印度植物性食物抗氧化活性的信息却很少。因此,本研究评估了印度常见食用的谷物、小米、豆类和豆科植物的抗氧化活性,并评估了其与总酚含量(TPC)的关系。通过DPPH(2,2-二苯基-1-苦基肼)自由基清除试验、铁还原抗氧化能力(FRAP)试验和还原能力来评估抗氧化活性。DPPH清除活性范围为0.24至1.73毫克/克,而FRAP范围为16.21至471.71微摩尔/克。龙爪稷(穇子)和菜豆的FRAP最高,分别为471.71、372.76,DPPH清除活性最高,分别为1.73、1.07。还原能力也观察到类似趋势。在谷物和豆类中,龙爪稷(拉吉)和黑绿豆(绿豆)的TPC最高,分别为373和418毫克/100克,而水稻(稻)和绿豆(金绿豆)的TPC最低(分别为47.6和62.4毫克/100克)。在本研究中,FRAP(r = 0.91)和还原能力(r = 0.90)与谷物和小米中的TPC呈显著相关,但与豆类和豆科植物中的TPC不相关。结果表明,TPC对印度谷物和小米的抗氧化活性有显著贡献。