用对流免疫电泳试验检测拟杆菌感染

Detection of Bacteroides infection by counter immunoelectrophoresis test.

作者信息

Lalitha M K, Mathai E, Elias L, Anandi V, Kalpana C R

机构信息

Department of Microbiology, Christian Medical College Hospital, Vellore.

出版信息

Indian J Med Res. 1991 May;93:171-3.

PMID:1937595

Abstract

The counterimmunoelectrophoresis (CIE) test using sonicated antigens of Bacteroides fragilis NCTC 2553 and a B. asaccharolyticus strain, standardised in the laboratory yielded a negative result in the 50 normal sera tested, while it was positive in 24 of 34 (71%) patients with infection due to black pigmented bacteroides and in 10 of 15 (67%) with B. fragilis infection. The microagglutination test (MAT) done in parallel showed a positivity of only 44 and 40 per cent respectively. The CIE test done with B. asaccharolyticus antigen was negative in 87 per cent of patients with infection due to B. fragilis whereas MAT showed cross reactivity to a greater extent.

摘要

使用脆弱拟杆菌NCTC 2553和一株解糖拟杆菌的超声处理抗原进行的对流免疫电泳（CIE）试验，经实验室标准化后，在检测的50份正常血清中结果为阴性，而在34例（71%）由产黑色素拟杆菌引起感染的患者中有24例呈阳性，在15例（67%）脆弱拟杆菌感染患者中有10例呈阳性。同时进行的微量凝集试验（MAT）阳性率分别仅为44%和40%。用解糖拟杆菌抗原进行的CIE试验在87%的脆弱拟杆菌感染患者中为阴性，而MAT显示出更大程度的交叉反应。