Baquet Z C, Williams D, Brody J, Smeyne R J
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 262 Danny Thomas Pl., Memphis, TN 38105-3678, USA.
Neuroscience. 2009 Jul 21;161(4):1082-90. doi: 10.1016/j.neuroscience.2009.04.031. Epub 2009 Apr 17.
The substantia nigra pars compacta (SNpc) is a compact brain structure that contains a variable distribution of cells in both medial to lateral and rostral to caudal dimensions. The SNpc is the primary brain structure affected in Parkinson's disease, where loss of dopaminergic neurons is one of the major hallmarks of the disorder. Neurotoxic and genetic models of Parkinson's disease, as well as mechanisms to treat this disorder, are modeled in the mouse. To accurately assess the validity of a model, one needs to be assured that the method(s) of analysis is accurate. Here, we determined the total number of dopaminergic neurons in the SNpc of the C57BL/6J mouse by serial reconstruction then compared that value to estimates derived using model-based stereology and design-based stereology. Serial reconstruction of the SNpc revealed the total number of SNpc dopaminergic neurons to be 8305+/-540 (+/-SEM). We compared this empirically derived neuron number to model based and design-based stereological estimates. We found that model based estimates gave a value of 8002+/-91 (+/-SEM) while design-based estimates were 8716+/-338 (+/-SEM). Statistical analysis showed no significant difference between estimates generated using model- or design-based stereological methods compared to empirically-derived counts using serial reconstruction.
黑质致密部（SNpc）是一种紧密的脑结构，其细胞在内外侧和前后维度上分布不均。SNpc是帕金森病中受影响的主要脑结构，其中多巴胺能神经元的丧失是该疾病的主要特征之一。帕金森病的神经毒性和遗传模型以及治疗该疾病的机制在小鼠中建立。为了准确评估模型的有效性，需要确保分析方法的准确性。在这里，我们通过连续重建确定了C57BL/6J小鼠SNpc中多巴胺能神经元的总数，然后将该值与使用基于模型的体视学和基于设计的体视学得出的估计值进行比较。SNpc的连续重建显示，SNpc多巴胺能神经元的总数为8305±540（±标准误）。我们将这个通过实验得出的神经元数量与基于模型和基于设计的体视学估计值进行了比较。我们发现基于模型的估计值为8002±91（±标准误），而基于设计的估计值为8716±338（±标准误）。统计分析表明，与使用连续重建得出的实验计数相比，基于模型或基于设计的体视学方法得出的估计值之间没有显著差异。
