Zwerdling Astrid, Delpino M Victoria, Pasquevich Karina A, Barrionuevo Paula, Cassataro Juliana, García Samartino Clara, Giambartolomei Guillermo H
Instituto de Estudios de la Inmunidad Humoral (CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
Microbes Infect. 2009 May-Jun;11(6-7):689-97. doi: 10.1016/j.micinf.2009.04.010. Epub 2009 Apr 17.
Human brucellosis is caused by infection with certain species of the genus Brucella and is characterized by bacterial persistence and inflammation of many host tissues. Neutrophils are one of the predominant cell types present in the infiltrate of these inflamed tissues, and due to their potential effect on the inflammatory response and tissue damage, direct activation of neutrophils by Brucella abortus might contribute to the pathology associated with human brucellosis. B. abortus expresses outer membrane lipoproteins (Omp) with inflammatory properties on a variety of cell types. This study examines the effect of B. abortus and its lipoproteins on neutrophil functions. B. abortus induced an increment in CD35 and CD11b expression and a decline in CD62L accompanied by IL-8 secretion, a response compatible with neutrophil activation. B. abortus lipoprotein Omp19 (L-Omp19), but not its unlipidated form, mimicked the changes associated with neutrophil activation induced by B. abortus. L-Omp19 primed neutrophils for oxidative burst as well as promoted neutrophil migration and prolonged neutrophil survival. Thus, Brucella lipoproteins possess pro-inflammatory properties that could contribute to the localize tissue injury and inflammation by direct activation of neutrophils. Data presented here, together with our previous results implicate Brucella lipoproteins in the pathogenesis of human brucellosis.
人类布鲁氏菌病是由感染布鲁氏菌属的某些菌种引起的，其特征是细菌持续存在以及许多宿主组织发生炎症。中性粒细胞是这些发炎组织浸润中存在的主要细胞类型之一，由于它们对炎症反应和组织损伤具有潜在影响，流产布鲁氏菌直接激活中性粒细胞可能会导致与人类布鲁氏菌病相关的病理变化。流产布鲁氏菌在多种细胞类型上表达具有炎症特性的外膜脂蛋白（Omp）。本研究考察了流产布鲁氏菌及其脂蛋白对中性粒细胞功能的影响。流产布鲁氏菌诱导CD35和CD11b表达增加，CD62L表达下降，并伴有IL-8分泌，这一反应与中性粒细胞激活相符。流产布鲁氏菌脂蛋白Omp19（L-Omp19），而非其未脂质化形式，模拟了流产布鲁氏菌诱导的与中性粒细胞激活相关的变化。L-Omp19使中性粒细胞引发氧化爆发，促进中性粒细胞迁移并延长中性粒细胞存活时间。因此，布鲁氏菌脂蛋白具有促炎特性，可通过直接激活中性粒细胞导致局部组织损伤和炎症。此处呈现的数据，连同我们之前的结果表明布鲁氏菌脂蛋白与人类布鲁氏菌病的发病机制有关。
