Mo Xiang-tao, Yang Zhi-ming, Qin Ting-wu W
Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, West China School of Clinical Medicine, Sichuan University, Chengdu 610041, China.
Bone. 2009 Aug;45(2):301-8. doi: 10.1016/j.bone.2009.04.200. Epub 2009 Apr 17.
To enhance osteointegration with preservation of mechanical strength, a surface modification technique using 20% surface demineralization in a controlled manner was applied to custom-built cylindrical bio-derived compact bones (20% surface-demineralized cylindrical compact bio-derived bone scaffold: SDCBS); an undemineralized version was the control. The micro-surface topography of the two types of bone scaffolds was characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). 20% demineralization led to significant increases in surface roughness (38.19%, P=0.001) and surface area (15.1%, P=0.030), compared with the control group's, while the decrease in mechanical properties was not statistically significant. Results of orthotopic implantation for 9 months demonstrated that 20% surface demineralization caused significantly rapid and homogeneous bone remodeling at the interface compared to control and led to a significantly rapid osteointegration of SDCBS with the host bone at the early and intermediate stages of osteointegration. The study indicates the potential of SDCBS in repairing clinical bone defects, and would help direct the use of various processes of biomaterials to support defect repairs within osseous sites.
为了在保持机械强度的同时增强骨整合，一种以可控方式进行20%表面脱矿质的表面改性技术被应用于定制的圆柱形生物衍生致密骨（20%表面脱矿质圆柱形致密生物衍生骨支架：SDCBS）；未脱矿质的版本作为对照。通过原子力显微镜（AFM）和扫描电子显微镜（SEM）对两种类型的骨支架的微观表面形貌进行了表征。与对照组相比，20%脱矿质导致表面粗糙度显著增加（38.19%，P=0.001）和表面积增加（15.1%，P=0.030），而力学性能的下降无统计学意义。9个月原位植入的结果表明，与对照组相比，20%表面脱矿质在界面处引起显著更快且均匀的骨重塑，并导致SDCBS在骨整合的早期和中期与宿主骨显著更快地骨整合。该研究表明SDCBS在修复临床骨缺损方面的潜力，并将有助于指导使用各种生物材料工艺来支持骨部位的缺损修复。
