Zheng Fu-ying, Lin Guo-zhen, Qiu Chang-qing, Zhou Ji-zhang, Cao Xiao-an, Gong Xiao-wei
Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
Res Vet Sci. 2009 Oct;87(2):211-2. doi: 10.1016/j.rvsc.2009.03.010. Epub 2009 Apr 18.
The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 microg/well and the dilution of serum was 1:20. It was optimal that sera with P/N >or= 2.2 were considered positive, P/N <or=2.0 negative, and between 2.0 and 2.2 ambiguous. The G(1)-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G(1)-ELISA may be a good alternative tool for seroepidemiological surveys.
牛暂时热病毒（BEFV）抗原位点G(1)的编码基因在宿主细胞大肠杆菌中得到高效表达。以重组蛋白作为包被抗原建立了间接G(1)-ELISA法，用于检测抗BEFV抗体。结果显示，包被抗原的最佳浓度为0.5μg/孔，血清稀释度为1:20。最佳判定标准为：P/N≥2.2为阳性，P/N≤2.0为阴性，2.0至2.2之间为可疑。通过检测590份田间血清样本，G(1)-ELISA法的敏感性为97.6%，特异性为98.6%。这些结果表明，G(1)-ELISA法可能是血清流行病学调查的一种良好替代工具。
