Brady L J, Piacentini D A, Crowley P J, Bleiweis A S
Department of Oral Biology, University of Florida, Gainesville 32610.
Infect Immun. 1991 Dec;59(12):4425-35. doi: 10.1128/iai.59.12.4425-4435.1991.
Eleven monoclonal antibodies (MAbs) specific for P1, the major protein surface antigen of Streptococcus mutans serotype c, were characterized by Western blot (immunoblot) analysis and by radioimmunoassay using whole bacterial cells. The approximate binding domains of the MAbs were determined by using full-length and truncated P1 polypeptides. The accessibility of these binding sites on the surfaces of intact bacteria was determined by radioimmunoassay. The ability of each MAb to cross-react with related proteins from strains of S. mutans serotypes e and f, S. sanguis, and S. sobrinus serotype g is also reported.
针对变形链球菌c血清型主要表面蛋白抗原P1的11种单克隆抗体（MAb），通过蛋白质印迹（免疫印迹）分析以及使用全菌细胞的放射免疫测定进行了表征。利用全长和截短的P1多肽确定了这些单克隆抗体的大致结合域。通过放射免疫测定确定了完整细菌表面这些结合位点的可及性。还报道了每种单克隆抗体与变形链球菌e和f血清型菌株、血链球菌以及远缘链球菌g血清型相关蛋白发生交叉反应的能力。
