Kost Joseph
Department of Chemical Engineering, Ben-Gurion University, Beer-Sheva, 84105, Israel.
Methods Mol Biol. 2009;536:173-9. doi: 10.1007/978-1-59745-542-8_19.
Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).
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