Kurien Biji T, Scofield R Hal
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA.
Methods Mol Biol. 2009;536:55-65. doi: 10.1007/978-1-59745-542-8_8.
Protein blotting is an invaluable technique in immunology to detect and characterize proteins of low abundance. Proteins resolved on sodium dodecyl sulfate (SDS) polyacrylamide gels are normally transferred electrophoretically to adsorbent membranes such as nitrocellulose or polyvinylidene diflouride membranes. Here, we describe the nonelectrophroretic transfer of the Ro 60 (or SSA) autoantigen, 220- and 240-kD spectrin antigens, and prestained molecular weight standards from SDS polyacrylamide gels to obtain up to 12 immunoblots from a single gel and multiple sera.
蛋白质印迹法是免疫学中一种用于检测和鉴定低丰度蛋白质的重要技术。在十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶上分离的蛋白质通常通过电泳转移到吸附膜上，如硝酸纤维素膜或聚偏二氟乙烯膜。在此，我们描述了Ro 60（或SSA）自身抗原、220-kD和240-kD血影蛋白抗原以及预染分子量标准物从SDS聚丙烯酰胺凝胶的非电泳转移，以便从一块凝胶和多份血清中获得多达12张免疫印迹。
